Fig. 9
DATs and [K+]o gate intracellular Ca2+ dynamics during paired-pulse stimulations. a Images of VTA and SNc from DAT-Cre:Ai95D mice showing GCaMP6f eGFP expression (green) in TH-positive neurons (red). b, d Examples of Ca2+-imaging responses (changes to GCaMP6f fluorescence, ΔF/F) (mean ± SEM from duplicate) in DA axon population imaged in CPu in response to single or paired electrical pulses at IPI of 10 and 40 ms in (b) 5 mM [K+]o (grey) or 7.5 mM [K+]o (black), or (d) in control conditions (black) or the presence of cocaine (5 µM) (blue). Data are rescaled to 1p in each condition to control for photobleaching. c, e Mean peak values for GCaMP6f ΔF/F evoked by 2 pulses vs IPI. Data are normalised to value for 1 pulse (N = 5 animals). Two‐way ANOVA with Fishers LSD test for post hoc comparisons: *P < 0.05, **P < 0.01, ***P < 0.001. DHβE (1 µM) is present throughout. Error bars are ± SEM. Source data are provided as a Source Data file
