Fig. 1
From: Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning
Both mU6 promoter and hU6 promoter enable to transcript gRNAs initiated with A or G for genome editing. a Schematic diagram of mU6 and hU6 comparison. These two promoters were used to transcript gRNAs initiated with A or G nucleotide. b, c Comparison of mU6 and hU6 promoters for genome editing with gRNAs initiated with G. The data are shown as mean ± s.d. (n = 2). d, e Comparison of mU6 promoter for genome editing with gRNAs initiated with A. The data are shown as mean ± s.d. (n = 2). f Comparison of the mU6 promoter for genome editing with gRNAs initiated with A or G three days post transfection. g Comparison of the mU6 promoter for genome editing with gRNAs initiated with A or G 5 days post transfection. The data are shown as mean ± s.d. P > 0.05; P < 0.05 by two-way ANOVA (n = 2). Source data are provided as a Source Data file
