Fig. 4
From: Optimized CRISPR guide RNA design for two high-fidelity Cas9 variants by deep learning
Influence of nucleotide composition on gRNA activity. a–c The position-dependent nucleotide composition of the highest 25% active gRNAs versus lowest 25% active gRNAs. Bars showed log-odds scores of nucleotide frequency for each position. The numbers below indicated the position of the nucleotides on-target DNA. d–f The association of each nucleotide number with gRNA activity. The size of the circles indicated indel frequency
