Fig. 1
Abnormally phosphorylated proteins in AR-97Q mice. a We analyzed the levels of phosphorylated proteins in the mouse model of SBMA which carries human androgen receptor (AR) with 97 glutamines (AR-97Q) at 6, 9, and 13 weeks of age and compared them to wild-type mice at the same ages using the Bio-Plex phosphoprotein assay (n = 3 animals per group). The p-values in each of the spinal cord and skeletal muscle were adjusted for false discovery rate (FDR) based on the Benjamini and Hochberg method to correct for multiple testing. *FDR < 0.1. b–e Immunoblots showing the levels of phosphorylated and total Src, Stat3 and p38MAPK proteins in the spinal cord (b) and skeletal muscle (c) of wild-type and AR-97Q mice at 6 weeks of age. The quantitative analysis was performed using densitometry (n = 3 mice per group) (d, e). f, g Immunohistochemistry for p-Src in the spinal cord (f) and skeletal muscle (g) of 6-week-old mice. h, i Immunohistochemistry for p-Src in spinal cord (h) and skeletal muscle (i) samples from control subjects and patients with SBMA. j Immunoblots for p-Src and Src in the biopsied muscle specimens of control subjects and patients with SBMA. Error bars indicate the s.e.m. Comparison between AR-97Q and wild-type groups was performed using the unpaired two-sided t-test. *p < 0.05 (d, e). SBMA, spinal and bulbar muscular atrophy; N.S., not significant; Wt, wild-type; AR, AR-97Q. Scale bars: 25 µm (f, g), 40 µm (h) and 20 µm (i). Source data are provided as a Source Data file (a–e, j)
