Fig. 5
Effect of A419259 on the histopathology of AR-97Q mice. a–d Immunohistochemical staining of the spinal cord (a, b) and skeletal muscle (c, d) from 13-week-old mice with an antibody against polyglutamine (1C2) and quantitative analysis of the staining. e, f Immunostaining for ChAT in spinal cord samples from 13-week-old mice. g, h HE staining of skeletal muscle (g) and quantitation of the muscle fiber size (h) in 13-week-old mice. Quantitative analyses were performed on sections from n = 3 animals per group. Error bars indicate s.e.m. Statistical analyses were performed using the unpaired two-sided t-test (b, d). **p < 0.01, ANOVA with Dunnett’s test (f, h). N.S., not significant. Scale bars: 25 µm (a, c, e, g). Source data are provided as a Source Data file (b, d, f, h)
