Fig. 6
From: The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications
Isolation of naive lymphocytes using an ALFA-tagged nanobody recognizing CD62L. Total human PBMCs were left untreated (Before sorting) or isolated using an ALFA SelectorPE resin loaded with an ALFA-tagged anti-human CD62L nanobody (After sorting). a A sketch of the affinity purification strategy. b Cells were stained with an anti-CD62L antibody and analyzed by flow cytometry. c The same cells as in b were stained with antibodies directed against CD3, CD19 and CD62L, and analyzed by flow cytometry. A forward scatter/side scatter gate was set on lymphocytes in all analyses (Supplementary Fig. 9). Color code used in a: NbALFA (orange), ALFA-tag (blue), CD62L (light gray), NbCD62L (dark gray), CD62L+ T-lymphocyte (yellow)
