Fig. 4 | Nature Communications

Fig. 4

From: DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates

Fig. 4

Actin polymerization drives integrin tension. a, c Representative before and after MT-FLIM images of NIH 3T3 fibroblasts on 4.7 pN MT-FLIM probes treated with 50 μM Y27632 or 0.5 μM jasplakinolide, respectively. b, d Average percent open probes in podosomes per cell before and after drug treatment. Blue and red lines represent an increase or decrease in percent open probes per cell, respectively. Gray horizontal lines represent the median percent open probes. Statistics were performed with a two-tailed Wilcoxon matched-pairs signed rank test. At least 34 cells from three experiments were analyzed per condition. ****P < 0.0001. All scale bars, 5 μm. Source data are provided as a Source Data file

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