Fig. 6
Podosomes maturation requires F > 12 pN integrin tension. a Schematic depicting integrin-TGT interaction on an SLB. First, an integrin receptor binds to a TGT duplex on the SLB. Upon FInt > Ttol, the TGT irreversibly ruptures, creating a transient local concentration gradient. Probe density is replenished through diffusion, and the cycle repeats. b Ttol is set by relative orientation of the cRGD ligand and the biotin anchor. c Representative images of podosomes on TGTs. Scale bar, 5 μm. d Cell area on TGTs. Teal bars represent the median area. Statistics were performed with a rank sum test. At least 332 cells were analyzed per condition, three experiments. e Percentage of cells forming podosomes on TGTs. Bars represent the mean ± s.d. (error bars), and teal circles represent individual experiments. Samples were compared using an unpaired two-tailed Student's t test. At least 76 cells were analyzed per surface, three experiments. f Scatter plot showing the number of podosomes per cell in podosome-forming cells on 12 versus 56 pN TGTs. Teal bars represent the median. Statistics were performed using a rank sum test. At least 25 cells were analyzed per condition, three experiments. Outliers more than three quartiles above or below the upper quartile were eliminated. g Scatter plot showing the mean podosome depletion on 12 versus 56 pN TGTs. Teal bars represent the mean depletion. Statistics were performed using a two-tailed unpaired Students t test. At least 25 cells were analyzed per condition, three experiments. Cells spread for 2 h on TGTs prior to imaging. **P < 0.01, ****P < 0.0001. Source data are provided as a Source Data file
