Fig. 1
Roles of B. bassiana bba-milR1 on fungal pathogenicity against mosquitoes. a bba-milR1 cannot be detected in B. bassiana Dicer2 deletion mutant (ΔDcl2) and Dicer1/Dicer2 double-mutant (ΔDcl1/Dcl2), as revealed by RT-PCR. RNA was extracted from fungal mycelium. b Survival of adult female A. stephensi mosquitoes infected with the wild-type (WT) ARSEF252 and two mutant strains milR1-KO 1# and milR1-KO 2# following topical application of a spore suspension (107 conidia/ml). Mosquitoes sprayed with sterile 0.01% Triton X-100 were used as a negative control (Triton). c Survival of female adult mosquitoes following topical application of a suspension of 107 conidia/ml of WT and two overexpression strains milR1-OV 1# and milR1-OV 2#. The statistical significance of survival curves was analyzed with the Log-rank test. d Expression of bba-milR1 during B. bassiana ARSEF252 infecting A. stephensi. RNA was extracted from fungus-infected mosquitoes. The B. bassiana U6 small nuclear RNA (U6) was used as an internal reference in qRT-PCR assays. The expression values are normalized to time 0. The data represent three biological repeats with three technical replicates and are shown as mean ± s.e.m. Source data are provided as a Source Data file
