Fig. 7
Tet-mediated DNA hydroxymethylation is associated with chromatin higher-order structures. a Representative histograms of compartment A and B distribution (top) calculated by Eigenvector using HiChIP and CMS-IP-seq (for 5hmC) data over chromosome 2 in E12.5 control embryonic heart tissues. b Scatterplot showing the distribution of Eigenvector calculated in control and Tet2/3-DKO embryonic heart tissues at E12.5. Pearson correlation coefficient was calculated. c Expression levels of genes that underwent A-to-B compartment switch in control and Tet2/3-DKO embryonic heart tissues (E12.5). d Representative GSEA analysis of genes located in genomic regions that showed A-to-B compartment switch in Tet2/3-DKO embryonic heart tissues (E12.5). e Histograms of PETs (distance and numbers) from HiChIP data obtained from control and Tet2/3-DKO heart tissues collected at E12.5. f Scatterplot showing the normalized numbers of PETs that overlapped with DEGs (regions ranging from TSS to TTS) in control and Tet2/3-DKO heart tissues collected at E12.5. g WashU Epigenome browser views of 5hmC, ATAC-seq, PETs (HiChIP), H3K4me1 (ENCODE) and H3K27ac (ENCODE) at the Tbx20 and Hey2 loci in control and Tet2/3-DKO heart tissues collected at E12.5
