Fig. 6

Sexual dimorphism in macrophages across tissues. a Pearson correlation matrix of MFs from the peritoneal cavity (PC-MF, green), spleen (Sp-MF, yellow), and microglia (labeled CNS, purple), based on 10,264 genes expressed above the noise threshold, sorted by cell type and sex. b Heatmap of the expression values of genes that are significantly contributing (two-way ANOVA, pFDR < 0.2, female–male fold change >log₂(1.5) in all three tissues) to sex effect across tissues (16 genes). c Heatmap of the expression values of genes that are significantly contributing to the sex–tissue interaction effect in specific tissue (two-way ANOVA pFDR < 0.2, male–female tissue-specific fold change >log₂(1.5)): left PC-MF, middle Sp-MF, 127 female and 101 male specific genes; right microglia (labeled CNS), 5 female and 43 male specific genes. In b, c, dotted horizontal white lines separate female from male upregulated genes. Dotted vertical white lines separate female and male samples. Values were normalized per tissue. Source data for a–c are provided as a Source Data file