Fig. 4

CAF-derived NLRP3 inflammasome signalling facilitates tumour growth. a Scheme of experiments analysed in b–h. shNlrp3 AT3 cells were orthotopically co-injected with WT (Nlrp3^+/+) or with Nlrp3 ^−/− NMFs (C57BL/6) into C57BL/6 mice. b Growth curves of AT3 tumours. n = 7 mice per group. c Tumour weights at termination of experiment. n = 6 and 7 mice per group (WT and Nlrp3^−/−, respectively). d Flow cytometry analysis of Gr1⁺ cell infiltration into AT3 tumours co-injected with WT NMFs (n = 4) or with Nlrp3^−/− NMFs (n = 3). Data presented are percentage of CD45⁺ cells, normalised to WT. e Representative images of staining with anti-Gr1 antibody in AT3 tumours. Scale bar, 100 μm. f Quantification of staining shown in d. Three fields of 3 mice/group were analysed; Data are presented as mean Gr1⁺ cells/field ± s.d; Mann–Whitney test. g, h Flow cytometry analysis of CD11b⁺Ly6C^high Ly6G⁻ and CD11b⁺Ly6G⁺Ly6C⁻ cell infiltration into AT3 tumours co-injected with WT NMFs (n = 4) or with Nlrp3^−/− NMFs (n = 3). Data presented are percentage of CD45⁺ cells, normalised to WT. i Scheme of experiments analysed in j–r. Met-1 cells were orthotopically co-injected with NMFs depleted of Nlrp3 or Il1b expression (shNlrp3, and shIl1b), or with control NMFs (shScramble). j Growth curve and k weight at endpoint of Met-1 tumours. n = 5, 10, 20, 19 tumours (TCs only, shScramble, shNlrp3, and shIl1b, respectively). l–n Flow cytometry analysis of Gr1⁺ cell infiltration into Met-1 tumours. n = 7, 14, and 16 per group (shScramble, shNlrp3, and shIl1b, respectively). Data presented are percentage of CD45⁺ CD11b⁺ cells, normalised to shScramble. o–r Met-1 tumours were analysed for the expression of selected genes using qRT-PCR. n = 6, 7, and 5–6 tumours per group (shScramble, shNlrp3, and shIl1b, respectively). Data are presented as fold change from shScramble. In b–d, g, h, j, k, data are presented as mean ± s.e.m; Welch’s t-test, *p < 0.05, **p < 0.005. In j–r data are presented as mean ± s.e.m; One-way analysis of variance test followed by Tukey’s multiple comparisons test. Data are representative of three independent biological experiments. Source data are provided as a Source Data file