Fig. 5
From: Engineering transferrable microvascular meshes for subcutaneous islet transplantation
Human iPSC-EC-derived microvascular meshes enhance vascularization. a Fluorescent images of iPSC-EC meshes with different geometries (square, pentagon, hexagon, and octagon). b iPSC-EC meshes with more complex patterns (spider web and capillary bed). c Confocal images of an iPSC-EC mesh at the contracted and junction regions. iPSC-EC expressing GFP is green, human CD31 antibody is red, and nucleus is blue. All samples were imaged after 2 days of culture. d Hematoxylin/eosin and immunostaining images of retrieved devices after 2 weeks of subcutaneous implantation in SCID-Beige mice. In all in vivo experiments, NHDFs were mixed with iPSC-ECs (iPSC-EC:NHDF = 9:1). The yellow arrowheads point to blood vessels with erythrocytes inside. The white dash lines mark the interface between the device and panniculus carnosus muscle. Mouse CD31 antibody is green and α-smooth muscle actin (α-SMA) is red. e The density and area percentage of blood vessels at the interface. n = 5 in all groups. Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and NS (P > 0.05) no significant difference. One-way analysis of variance. f Immunostaining images of human (red) and mouse CD31 (green) antibodies showing the anastomoses between iPSC-EC derived vessels and mouse vessels
