Fig. 6
Association of 21-nt risiRNAs with AGO1. a Length distribution of mapped reads from sRNA-seq of AGO1 IP in WT and fry1-6. The proportion of 21-nt reads in all mapped reads is slightly higher in fry1-6 than in WT. b Genomic classification of 100-bp bins with an enrichment of 21-nt sRNAs in AGO1 IP vs. input from WT and fry1-6. Although the numbers of bins corresponding to miRNAs and ta-siRNAs are similar between WT and fry1-6, those of bins corresponding to coding genes and rRNAs dramatically increased in fry1-6. The annotation was adopted from known genome features. c Distribution of 5′ nucleotides among 21-nt sRNAs derived from rRNA regions. In WT, a 5′-U preference was only observed among reads from the antisense strand in AGO1 IP, while in fry1-6, a 5′-U preference was observed among reads from both strands. This discovery suggests that the majority of sense reads in WT are not bound by AGO1, while those in fry1-6 can be loaded into AGO1. d Venn diagrams for genes with enriched 21-nt sRNAs. In AGO1 IP, most genes with enriched 21-nt sRNAs identified in WT were also identified in fry1-6. The 1224 genes in fry1-6 also included most of the 21-nt hyper DSGs identified in fry1. Source data are provided as a Source Data file
