Fig. 1
From: Glycan-mediated enhancement of reovirus receptor binding
Probing reovirus binding to living cells. a Schematic of reovirus particles with outer-capsid proteins labeled before (virion) and after (infectious subvirion particle [ISVP]) proteolytic processing. The cartoon shows the arrangement of outer-capsid proteins in the double-layered shell of virions, the formation of ISVPs by removal of σ3 and cleavage of µ1 to yield δ and ϕ, and rearrangement of σ1 into a more elongated conformation. b Full-length model of reovirus σ1 protein28, which functions as the viral attachment protein that binds to cell-surface glycans (in particular, to terminal α-linked sialic acid [α-SA] residues) and junctional adhesion molecule-A (JAM-A). Regions of the molecule that interact with α-SA and JAM-A are indicated. c Schematic of probing reovirus entry using AFM. The initial attachment of reovirus to cells involves specific binding between the viral σ1 protein and the receptor, JAM-A. Cell-surface glycans serve as attachment factors, and virus binding to their α-SA groups function in the initial association of virus to cells and further facilitates high-affinity binding to JAM-A
