Fig. 6
From: Glycan-mediated enhancement of reovirus receptor binding
Influence of sialylated glycans on reovirus binding to JAM-A. a Binding of T3SA+ or T3SA− virions, or T3SA+ ISVPs to JAM-A was monitored following injection of 1 mM α-SA glycans (Neu5Ac [b, red] and LSTa [c, yellow]) or non-sialylated glycan (LNnT [d, green]). b–d DFS plots of interaction forces measured between T3SA+ and JAM-A after adding 1 mM glycan (Neu5Ac in b, LSTa in c, and LNnT in d). Grey dots represent the measured binding forces before injection. e DFS plot of the interaction forces between JAM-A and T3SA+ ISVPs, which display a more extended conformation of the σ1 protein. Multivalent interactions are observed for T3SA+ ISVPs (blue) in comparison to T3SA+ virions (gray) without injection of free SA. f Example for analysis of the number of established bonds shown in panel g. Relative frequency of single and multiple bonds before and after adding free glycans was determined from areas under respective peaks (AI, AII, AIII; grey shaded) within force distribution histograms (red bars with cumulative peak, ATOT). g Number of bonds established between JAM-A and T3SA+ (left panel) or T3SA− (middle panel) virions or T3SA+ ISVPs (right panel), before and after injection of sialylated (Neu5Ac—red, LSTa—yellow) or non-sialylated (LNnT—green) glycans. Error bars indicate s.d. of the mean value. For all experiments, data are representative of at least n = 3 independent experiments. Source data are provided as a Source Data file
