Fig. 1
From: A fiber-deprived diet disturbs the fine-scale spatial architecture of the murine colon microbiome
The colon microbiome is compartmentalized. a Sampling design. The entire colon was cryopreserved and then subsampled in seven consecutive blocks (1 cm each) along the colon. At least four areas from each section-block were sampled with the laser microdissection microscope. b Similarity (Bray–Curtis distance) of microbiota composition to stool in CON diet from proximal to distal colon. Interpolated average values are shown. c Variation partitioning analysis, which shows the percentage of variation in microbiota composition attributable to longitudinal axis (from proximal to distal colon) and lateral axis (lumen and mucus). d Alpha diversity heatmaps show an increase of ASV richness and Shannon diversity in the lumen compared to mucus and an increase from proximal to distal colon (n = 211, ANOVA and Tukey’s post hoc test, mid-colon—distal colon p < 0.001, proximal colon—distal colon p = 0.0027). Interpolated average values are shown
