Fig. 3
From: A fiber-deprived diet disturbs the fine-scale spatial architecture of the murine colon microbiome
Deprivation of dietary fiber and polysaccharides alters the intestinal metabolite landscape. a PCA ordination illustrating that diet affects the caecal metabolite profile, as determined by global mass spectrometry-based metabolomics experiments. CON = blue, FD = orange, PFD = green. b Boxplots of selected metabolites in mouse caecum contents (in arbitrary peak intensity units). Boxplot: boxplot medians (center lines), interquartile ranges (box ranges), whisker ranges. (ANOVA: Propionic acid: p = < 0.0001, n = 16; butyric acid: p < 0.0001, n = 16). c Nanoparticle imaging mass-spectrometry of colon sections, demonstrating a decrease in propionate and butyrate in FD and PFD diets. Both propionic and butyric acid were analyzed as the K+ adduct at 113.0000 and 127.0156 m/z, respectively. Cell membrane was visualized by the mass of phosphocholine (yellow) at 184.0733 m/z. The scale for each analyte represents binned signal intensity for each 50 µm spot
