Fig. 6
From: A fiber-deprived diet disturbs the fine-scale spatial architecture of the murine colon microbiome
Diet-dependent spatial fragmentation of the microbiota. a Taylor’s power analysis of the heterogeneity of microbiota composition (ANOVA, lumen vs. mucus p = 0.004, n = 18). CON = blue, FD = orange, PFD = green, P = proximal colon, M = middle colon, D = distal colon. Boxplot: boxplot medians (center lines), interquartile ranges (box ranges), whisker ranges. b FISH quantification of patchiness of Lachnospiraceae. The density probability with respect to cluster area (µm2) in both lumen and mucus is shown. A representative FISH image shows a typical aggregation pattern in the distal colon in PFD diet Lachnospiraceae (pink), all Bacteria (green) and DAPI DNA staining (blue) are shown. CON = blue, FD = orange, PFD = green, solid line = lumen, dashed line = mucus. Metagenome analysis: Gene richness (ANOVA, p = 0.0018, n = 9) (c) Bray–Curtis distance (ANOVA, p = 0.0055, n = 9) (d) of samples from the same condition, and the average number of unique genes per KEGG functional category (ANOVA, p = 0.0024, n = 9) (e) for FD and CON are shown. CON = blue, FD = orange, PFD = green, P = proximal colon, M = middle colon, D = distal colon. Boxplot: boxplot medians (center lines), interquartile ranges (box ranges), whisker ranges. Source data are provided as Source Data file
