Fig. 5 | Nature Communications

Fig. 5

From: Disruptive mutations in TANC2 define a neurodevelopmental syndrome associated with psychiatric disorders

Fig. 5

rols is an essential gene expressed in the developing and adult nervous system of flies. a Schematic of the rols locus where the rolsMI02479-TG4.1 allele was generated by genetic conversion of the y1 w* Mi{MIC}rolsMI02479 allele via RMCE using ΦC31 expression to swap the original MiMIC insertion cassette for an SA-T2A-GAL4-polyA. The SA-T2A-GAL4 in the first intron of rols acts as an artificial exon resulting in early truncation of the rols transcript due to the polyA sequence. During translation, the T2A promotes ribosomal skipping and subsequent translation of GAL4 under the endogenous regulatory elements of rols. b Homozygous rolsMI02479-TG4.1 mutant flies are embryonic lethal. Embryonic lethality is also observed in rolsMI02479-TG4.1/Df(3L)ED4475, which can by rescued by introduction of a 80 kb genomic rescue construct inserted on chromosome 2 (VK37). c Larval and adult brain staining (GFP) of rolsMI02479-TG4.1>UAS-mCD8::GFP flies reveals membranous staining throughout the brain at both stages. Scale bar = 50 μm

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