Fig. 4

AgRP neurons connect to parts of the glutamatergic Vglut2-expressing neurons in the LHA. a Representative immunohistochemical image of a coronal section of AgRP axon terminals (green) and Vglut2 neurons (red) in the LHA. Vglut2 neurons were labeled with mCherry by injection of AAV-FLEX-tdTomato in the LHA of Vglut2-ires-Cre mice. b Schematic image of the monosynaptic rabies tracing of Vglut2 neurons in the LHA (left) and a brain section after monosynaptic-tracing neurons in the ARC that are synaptically connected to Vglut2^LHA neurons in the ARC (right). c Enlarged view of ARC neurons that are monosynaptically connected to Vglut2^LHA neurons (green) and AgRP immunostaining (red) in the ARC. Arrows show parts of AgRP neurons monosynaptically connected to Vglut2^LHA neurons