Fig. 1 | Nature Communications

Fig. 1

From: Human placental trophoblast cells contribute to maternal–fetal tolerance through expressing IL-35 and mediating iTR35 conversion

Fig. 1

IL-35 is present in the human serum and trophoblast cells. a The serum from early pregnant women (left, n = 17) and age matched non-pregnant women (right, n = 13) were collected and IL-35 level was measured by ELISA. The detection range of IL-35 was 62.5–4000 pg ml−1. b The mRNA level of the two subunits of IL-35 (ebi3 and p35) in primary trophoblasts (PT) and HTR8 cells were detected by quantitative real-time RT-PCR analysis. The results were normalized to endogenous control (GAPDH) (n = 2). c ELISA of IL-35 content in the supernatant of HTR8 cells (n = 3). d Immunocytochemical staining of EBI3 and p35 in PT and HTR8 cells. Scale bar: 50 μm. e Immunofluorescence staining of EBI3 (red) and p35 (green) in HTR8 cells. Scale bar: 20 μm. Data in 1a were represented as median with interquartile range. Significance was determined by Mann–Whitney U-test. Data in 1b and 1c were represented as mean ± SD. Significance was determined by Student’s t test analysis. ***P < 0.001

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