Fig. 5

Phenotype of fetuses from NP mice and AP mice and the expression of IL-35 in the placenta. Pregnant CBA/J female mice were euthanized on Day 13.5 of pregnancy. a–d Representative pictures showing the embryos and live fetuses per uterus from different treated AP and NP mice. Red arrows: aborted embryos. e Embryos size were measured. From left to right: n = 35 embryos from four separate mothers (AP mice), n = 39 embryos from four separate mothers (NP mice), n = 38 embryos from four separate mothers (AP mice + vehicle), n = 31 embryos from four separate mothers (AP mice + IL-35), n = 25 embryos from three separate mothers (AP mice + vehicle), n = 27 embryos from three separate mothers (AP mice + IL-35 mAb), n = 28 embryos from three separate mothers (NP mice + vehicle) and n = 25 embryos from three separate mothers (NP mice + IL-35 mAb). f Proportion of live embryos per uterus was calculated with following formula: Proportion of live embryos per uterus = (Number of live embryos per uterus)/(Number of total embryos per uterus). g–h Pregnant CBA/J female mice were euthanized on Day 13.5 of pregnancy and the placenta was isolated and teased apart. Then the protein and mRNA were extracted from the tissue. The protein levels of EBI3 and p35 in placenta were determined by western blot (n = 4) (g). Quantitative real-time RT-PCR analysis was applied to compare the expression of ebi3 and p35 in the placenta of NP and AP females (n = 4) (h). Data were represented as mean ± SD. Significance was determined by Student’s t-test. *P < 0.05, **P < 0.01, and ***P < 0.001