Fig. 3
From: Fusion dynamics of cubosome nanocarriers with model cell membranes
Dynamic analysis of individual cubosome fusion. a TIRF snapshots of a single cubosome fusion event over the course of ~6 s. Scale bar = 1.5 µm. b The corresponding plot of intensity with time during a single fusion event. The best fit to I = at-n is shown by the dashed line; for this case a ~2, n ~0.23. c Plot and distribution of fusion time and fitted exponent for many MO cubosome fusion events on a DOPC bilayer. The mean exponent, proposed 1/6 exponent and theoretical 2D diffusion exponent are shown by the solid black, dashed black and solid red lines, respectively. d Distribution of fusion time and fitted exponent for 1% DOTAP cubosomes with 0% DOPS, 40% DOPS and 40% DOPS 30% CHOL membranes. e Distribution of fusion times and fitted exponents for 1% DOTAP cubosomes with 40% DOPS and 40% DOPS 30% CHOL bilayers in PBS. f Measured mean-squared displacement for cubosomes above SLB. The dashed line is best fit against <r2> = 2d Dt. The determined particle sizes from each slope are shown in the legend. g DLS particle size distribution for cubosomes with increasing DOTAP composition. The corresponding zeta potential (ζ) measurements are shown in the inset
