Fig. 2

Enhanced neuropeptide visualization via nsPCR. a Venn diagrams showing two sets of comparative data for both lipid and neuropeptide identifications with and without NBA treatments. b Optical images of mouse brain for control and experimental group with NBA. c Direct comparison of putative neuropeptide ADKNFLRFamide ([M + H]⁺, m/z 1009.568) without (left panel) and with NBA treatment (right panel); d APQRNFLRFamide ([M + H]⁺, m/z 1147.650); e RKPPFNGSIFamide ([M + K]⁺, m/z 1199.610); f RSAEGLGRMGRL ([M + K]⁺, m/z 1340.660). g Direct comparison of lipid (triacylglycerol, [TAG(52:9) + Na]⁺, m/z 867.651) distributions in mouse brain between control and labeled group with NBA. h Typical distribution of lipid (monomethyl-phosphatidylethanolamine, [MMPE(44:10) + 34 + Li]⁺, m/z 878.645) with NBA. i Overlay of lipid (phosphatidylcholine, [PC(36:1) + H]⁺, m/z 788.615, red) and neuropeptide ([SKNYLRFamide + H]⁺, m/z 926.522, green) ion images. All images were obtained with m/z tolerance of 5 ppm. Scale bar, 2 mm. Step size, 50 μm