Fig. 4
Large protein labeling via nsPCR. a Correlation between average number of nsPCR-crosslinked NBA and the molecular weight of large proteins. b Correlation between average number of nsPCR-crosslinked NBA and the total number of free amine groups on large proteins. Cyt c, cytochrome c; HSA, human serum albumin; bTF, bovine transferrin. The proteins in control group are preserved with 100 mM ammonium acetate, while unfolded proteins are obtained through heating in 100 mM ammonium acetate at 95 °C for 10 min. The error bars represent S.D. with n = 3 biologically independent experiments. c The structural effects of terminal sialylation on glycoprotein mouse transferrin (TF) probed by nsPCR. As a control (de-sialylated), two sialic acids (Neu5Gc, mass shift between peaks 1 and 2) on mouse transferrin are removed with sialidase under the native condition. The number of surface accessible amine group for sialylated (mass shift between 1 and 3) and de-sialylated (mass shift between peaks 2 and 4) transferrin is probed as 14.4 ± 1.1 (n = 3) and 20.7 ± 1.3 (n = 3), respectively. W/O, without; W/, with. Source data is provided in a Source Data file
