Fig. 2

Dynamic redistribution of BRD4 between HSCP-HKs and LSCP-HKs. a Ranking of all active genes by change in BRD4 occupancy at their promoter and proximal enhancers between HSCP-HK and LCSP-HK, with fold change in BRD4 occupancy shown in top panel. In middle and bottom panels, fold change in H3K27ac and mRNA levels are shown using a binned average (100 genes per bin), respectively. Error bars represent 95% confidence intervals (CI) of the average as determined by resampling with replacement (1000 permutations). A trend line (black) is overlaid by lowest locally weighted regression. b Transcript levels of RBP1 and ZFP42 based on RNA-seq (FPKM) comparing HSCP-HK to LSCP-HK. Error bars represent standard deviation. c Chromatin occupancy of BRD4 and H3K27ac (ChIP-seq) as well as chromatin accessibility (ATAC-seq) at RBP1 and ZFP42 genomic loci as read density in units of reads per million per base pair (rpm/bp). Individual HSCP-HK (blue) and LSCP-HK (red) replicates are shown as shaded traces. The average of replicates is drawn as a solid line