Fig. 2
From: Transforming protein-polymer conjugate purification by tuning protein solubility
Ammonium sulfate precipitation of conjugates. a, b Native Lyz, Lyz(5+), and Lyz–polymer conjugates. Plots are solubility (log of the supernatant protein concentration) versus ammonium sulfate percent saturation. 100% saturation corresponds to 4.1 M salt concentration. a Lyz(5+)pCBMA conjugates with DP 18, DP 32, DP 56, DP 79, and DP 91. b Lyz(5+)pOEGMA conjugates with DP 25, DP 43, DP 90, DP 105, and DP 164. pCBMA increased Lyz’s solubility while pOEGMA decreased Lyz’s solubility depending on DP. c, d Ammonium sulfate precipitation of native Lyz(1+), Lyz(3+), and Lyz–polymer conjugates with lower grafting densities and low/high DP. c pCBMA conjugates of Lyz(1+) DP 14, Lyz(1+) DP 44, Lyz(3+) DP 20, and Lyz(3+) DP 66. The only pCBMA conjugate that precipitated was the lowest grafting density and lowest DP. d pOEGMA conjugates of Lyz(1+) DP 9, Lyz(1+) DP 93, Lyz(3+) DP 16, and Lyz(3+) DP 57. pOEGMA length affected solubility more than grafting density. Error bars represent the standard deviations from triplicate measurements (n = 3)
