Fig. 3
From: Epi-illumination gradient light interference microscopy for imaging opaque structures
Two challenging samples for transmission DIC a Reflected light imaging avoids the meniscus and high walls that make imaging microplates difficult. Representative field of view (×10/0.3) from a 1536 microplate. b Birefringent materials such as plastic substrates are incompatible with polarization-based contrast. This problem is overcome in epi-GLIM, by collecting the light before it passes through the birefringent material, revealing intracellular detail such as nucleoli (red arrow). Neurons were imaged after 7 days in vitro (×100/0.75 LD)
