Fig. 2

Global Tmem127 deficiency disrupts hepatic lipogenesis. a Relative iWAT mRNA expression (log) of the indicated fatty-acid synthesis, storage, oxidation, and transport gene expression in adult WT and KO mice (n = 4–5 per genotype) measured by real-time PCR (RT-PCR) and normalized to TfIIb gene; b western blots of fatty acid synthetase (FASN) and loading control (total AKT) in whole-tissue lysates prepared from iWAT from adult male WT or KO mice (n = 6 per genotype); c hepatic triglyceride content in fed adult WT and KO mice (n = 10–12 per genotype); d relative liver mRNA expression levels (log) of the indicated fatty-acid synthesis, storage, oxidation, and transport gene expression from adult WT and KO mice (n = 4–9 per genotype) measured by RT-PCR. e Western blots of Acetyl-CoA carboxylase (ACC), FASN and loading control (total AKT) in whole-tissue lysates prepared from liver from WT or KO adult male mice (n = 6 per genotype); data were analyzed by Student’s t test. Values are expressed as mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001. Adult mice were 9–12 months of age. See also Supplementary Fig. 2A–C. Source data are provided as a Source Data file