Fig. 8

Effects of TMEM127 deletion in hepatic gluconeogenesis and lipogenesis are cell autonomous. a Western blot of lysates from control (C) or CRISPR-Cas9 mediated- TMEM127 knockout KO HepG2 human hepatic cells treated with or without 100 nm insulin for 10 min, probed with Akt, TMEM127 or a loading control antibody; b real-time (RT) PCR of G6PC (the G6Pase gene) expression in control or TMEM127 KO HepG2 cells deprived of glucose and stimulated with pyruvate 2 mm (three biological repeats); c RT-PCR mRNA expression of the indicated fatty-acid synthesis, transcription factors, and glucose transporter genes from HepG2 control or TMEM127 KO cells (three biological repeats); d RT-PCR of fatty-acid synthesis or transcription factors from primary hepatocytes from Tmem127 WT or KO (Tm-CMV-KO); 12-week old-female mice, n = 3 per genotype). e Western blots of indicated phosphorylated and total proteins from whole-cell lysates of HepG2 TMEM127 KO or control cells with or without Rictor knockdown (Rict) and treated with insulin as indicated. Shown is one of four replicate experiments. Data were analyzed by Student’s t test. Values are expressed as mean ± s.e.m. *P < 0.05; **P < 0.01. See also Supplementary Fig. 8. Source data are provided as a Source Data file