Fig. 2
From: Oligomeric state of the ZIKV E protein defines protective immune responses
ZIKV rED induces neutralizing antibody responses. a Mice were primed with 5 µg of ZIKV rEM or rED (+/−alum) and boosted with similar doses at weeks 3 and 9. Mice were bled (b) at indicated time points (n = 5 for rEM, rED, rEM+alum, and rED+alum, and n = 3 for vehicle control). b ZIKV-specific single point dilution (1:50) IgG titers were determined by virus capture ELISA. c ZIKV-specific endpoint dilution (EPD) titers were determined at week 16 by virus capture ELISA; EPD is the serum dilution at which detection signal equals background levels. d ZIKV-neutralizing activity at week 16 was determined on Vero cells and expressed as the serum dilution at which 50% of the virus was neutralized (Neut50). Statistical differences were determined by one-way ANOVA followed by a Tukey’s test (*p < 0.05). Data points represent individual mice. Error bars represent standard deviation. Source data are provided as a Source Data file
