Fig. 1

Remodeling of the RORC promoter during thymocyte development. a Scheme of the human RORC locus: transcription from the RORC promoter generates the RORγ isoform; the RORC2 promoter generates the RORγt isoform. Blue boxes: unique RORC exons; pink box: unique RORC2 exon; purple boxes: common exons. Arrows indicate the primers used for RT-qPCR amplification of the two isoforms. b RORC and RORC2 expression in sorted thymocyte populations, and naive CD4⁺ or CD8⁺ T cells isolated from cord blood was quantified by microarray. Shown is average and standard deviation of five independent experiments. c Epigenetic modifications at the RORC, RORC2 and CD4 promoters. ChIP was performed with antibodies against histone 4 acetylation (H4Ac, top); histone 3 lysine 27 trimethylation (H3K27me3, middle) and histone 3 lysine 4 trimethylation (H3K4me3, bottom), on sorted thymocyte populations, and in naive CD4⁺ T cells from cord blood, followed by RT-qPCR of the RORC promoters and the CD4 promoter (as a quality control). ChIP with an irrelevant IgG antibody tested the specificity of binding (white bars). Data are expressed as percent of input and represent average and SD of three replicates. Source data are provided as a Source Data file