Fig. 2
From: NFAT primes the human RORC locus for RORγt expression in CD4+ T cells
Global remodeling of the RORC locus during thymocyte differentiation. a Alignment of the human and murine RORC loci, obtained with the VISTA browser. The Y-axis displays the percentage of identity between sequences. Regions with >70% identity are marked in color: Conserved Non-coding Sequences (CNS) in salmon, 5′ and 3′ untranslated regions (UTR) in light blue and exons in purple. The arrowheads show the localization of the CNS analyzed in the ChIP assays. CNSs are labeled according to their distance from the RORC2 Transcription Start Site. b The diagram on the left shows the organization of the RORC locus. The genomic regions amplified are marked with arrowheads. Enrichment of H3K4me3 (left panels), H3K27me3 (middle panels), and H4Ac (right panels) was analyzed by ChIP assay in DN, ISP, DP CD3low, DP CD3 intermediate, SP CD4 thymocytes, and naive CD4+ T cells from cord blood. Average and SD of three replicates are represented. Source data are provided as a Source Data file
