Fig. 5
From: Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
Live-cell imaging of the microtubules in U2OS cells. a, b 3D-pSIM images, obtained at 0.67 reconstructed fps, of the microtubules in a live U2OS cell (80 × 80 × 0.75 μm3) expressing tubulin-GFP. The maximum intensity projection (MIP) images for PM (a) and pSIM (b) are compared. 3D-pSIM images the in-plane dipole orientation together with doubled lateral and axial resolution as that of PM. c Time-lapse 2D sections of the 3D-pSIM results. d Schematic of the α-tubulin-GFP structure. The orientation of the ensemble dipole is perpendicular to the microtubule filament. e–g The ensemble dipole consists of a 2D in-plane projection of all the 3D GFP dipoles. The relative orientation between the GFP dipole and the α-tubulin monomer determines whether the ensemble dipole is perpendicular or parallel to the filament. If the included angle α between the GFP dipole and the microtubule filament is close to 90° (or 0°), then the averaged dipole orientation is perpendicular (or parallel) to the filament. Scale bars: a 10 μm and (b) 1 μm
