Fig. 3
From: Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores
DNA sensing by various engineered pores. a Structure of dA4 with its electrostatic potential mapped on the molecular surface. b Raw single-channel recording traces upon dA4 addition into the cis chambers of wt, R282A, R220A, R220W, K238A, K238Q, K238N, and K238R mutants, respectively. The final concentration of dA4 in the chamber was 2.0 μM. c Dwell time distribution of dA4 events of the wt (black, 5000 events), R220A (red, 5000 events), R220W (orange, 3200 events), K238A (yellow, 3800 events), K238Q (green, 4200 events), K238N (sky blue, 5000 events), and K238R (blue, 5000 events) mutants under +100 mV voltage. d Comparison between dwell time of dA4 (red triangles) and the pore diameter at the R220 (black circles) and K238 (blue circles) regions. e Electrostatic potential maps of wt, R282A, R220A, R220W, K238A, K238Q, K238N, and K238R along the pore lumen. The lipid membrane region is represented by a gray shadow, and the R282, R220, K238, and K242 regions are marked with the colors scheme used along the manuscript figures (see Fig. 1)
