Fig. 5
From: Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores
Positively charged peptide sensing by various engineering pores. a 3D structure of HIV-1 Tat (47–57) (PDB:1TAC) with its electrostatic potential mapped on the peptide molecular surface. b Raw single-channel recording traces upon HIV-1 Tat (47–57) addition into the cis side of the chamber for wt, R282A, R220A, or R220W, respectively. The final concentration of HIV-1 Tat (47–57) in the chamber was 2 μM. c Inter-event interval (τon) distribution of HIV-1 Tat translocation on the R282A (orange), R220A (green), and R220W (blue) pore mutants. The values were determined by the single exponential fitting. Relative dwell times are reported in Supplementary Fig. 16. d Raw current traces of HIV-1 Tat (47–57) translocation through R220W at different voltages ranging (from −60 mV to −100 mV). e Comparison between dwell time of HIV-1 Tat (47–57) peptide (orange squares) and the diameter of R220 (black circles) and K238 (blue circles) region
