Table 1 List of antibodies, Drosophila lines, oligonucleotides and reagents used in this study

Anti-TJ

Guinea Pig

1:4000

Gift from D. Godt¹⁸

Anti-Engrailed 4D9

Mouse

1:50

Development Studies Hybridoma Bank, University of Iowa

Anti-pMad

Rabbit

1:500

⁴⁵

Anti-GFP

Chicken

1:2000

Abcam Ab13970

Anti-GFP

Rabbit

1:4000

Invitrogen A6455

Anti-RFP

Rabbit

1:8000

Gift from S. Heidmann⁴⁶

Anti-myc 9E10

Mouse

1:40

Development Studies Hybridoma Bank, University of Iowa

Anti-eve 3C10

Mouse

1:40

Development Studies Hybridoma Bank, University of Iowa

Anti-eve

Rabbit

1:200

Gift from M. Frasch⁴⁷

Anti-vGlut (C-ter)

Rabbit

1:1000

Gift from H. Aberle⁴⁸

Anti-Gad1 818

Rabbit

1:500

Gift from F.R. Jackson⁴⁹

Anti-Repo 8D12

Mouse

1:40

Development Studies Hybridoma Bank, University of Iowa

Anti-Prospero

Mouse

1:40

Development Studies Hybridoma Bank, University of Iowa

Anti-FasII 1D4

Mouse

1:80

Development Studies Hybridoma Bank, University of Iowa

Anti-5-HT

Rabbit

1:2000

Immunotech ref. 0601

Anti-Jumu

Rabbit

1:800

Gift from J. Enriquez⁵⁰

Anti-guinea pig A488

Goat

1:1000

Alexa Fluor^TM (Invitrogen)

Anti-guinea pig A555

Goat

1:2000

Anti-guinea pig A647

Donkey

1:1000

Anti-chicken A488

Goat

1:1000

Anti-mouse A488

Donkey

1:1000

Anti-mouse A405

Goat

1:1000

Anti-mouse A555

Donkey

1:2000

Anti-mouse A647

Donkey

1:1000

Anti-rabbit A488

Donkey

1:1000

Anti-rabbit A555

Donkey

1:2000

Anti-rabbit A647

Donkey

1:1000

Other reagents

Phalloïdin-FluoProbes 647

NA

1:25

FP-BA0320, Interchim

TJ-Gal4

Tokyo Stock Center

DGRC # 104055

TJ-Flp

Generated by the lab

NA

Isl ^{− Ƭmyc}

²⁷

Gad1-LexA

Bloomington Stock Center

BL # 60324

vGlut-LexA

Bloomington Stock Center

BL # 60314

ChAT-LexA

Bloomington Stock Center

BL # 60319

UAS-TrpA1

Bloomington Stock Center

BL # 4308

UAS-shi ^ts

²⁰

NA

lexAop > stop > dTrpA1 (2 lines on II^d and III^d chromosomes)

Obtained from the Rubin lab (Janelia Research Campus)

NA

Per-LexA

Tokyo Stock Center

DGRC # 116999

Per-Gal4

Bloomington Stock Center

BL # 7127

FkhGFP

Bloomington Stock Center

BL # 43951

Tsh-LexA

Gift from J. Simpson (UC Santa Barbara)⁴¹

NA

Tsh-Gal80

Gift from G. Miesenböck (University of Oxford)²²

NA

lexAop-IVS-tdTomato.nls

Bloomington Stock Center

BL # 66680

UAS-H2AGFP

⁵¹

NA

20XUAS-6XGFP

Bloomington Stock Center

BL # 52262

Act»Gal4

Bloomington Stock Center

BL # 4779

UAS-CD8GFP

Bloomington Stock Center

BL # 5137

CQ2-LexA

Gift from C. Doe (University of Oregon)¹³

NA

sim-Gal4 (or sim3.7-Gal4)

Bloomington Stock Center

BL # 9150

Hlh3bGFP

Gift from P. Tomancak (Max Planck Institute) (unpublished)

NA

Grain-lacZ

⁵²

NA

Gad1 ^AD

Bloomington Stock Center

BL # 60322

UAS-LexA ^DBD

Bloomington Stock Center

BL # 56528

UAS-myrGFP

Bloomington Stock Center

BL # 32198

B-H1-Gal4

^{53, 54}

NA

JRC-SS00863 (split Gal4 for Ladder-d)

¹⁷

Tdc2-Gal4

Bloomington Stock Center

BL # 9313

Dimm-Gal4

Bloomington Stock Center

BL # 25373

TH-Gal4 (also called ple-Gal4)

Bloomington Stock Center

BL # 8848

vmatGFP

Bloomington Stock Center

BL # 60263

UAS»TrpA1 ^myc

Bloomington Stock Center

BL # 66871

lexAop-Flp

Bloomington Stock Center

BL # 55820

lexAop > stop > CsChrimson^Venus

Obtained from Rubin lab (Janelia Research Campus)

NA

GMR47G08-LexA

Bloomington Stock Center

BL # 52793

kanR

2xTY1-kanR

Gift from P. Tomancak (Max Planck Institute)²³

Late SV40 pA

pCAGGS-Ires2-eGFP-linkerPacI-sv40pA

Gift from J.F. Brunet (ENS Paris)

nlsFlpO

pPGK FlpO bpA

Gift from S. Bourane, Goulding lab (Salk Institute)

pFly-fosmid-TJ

NA

Gift from P. Tomancak²³

pRed-Flp4

NA

recTJsens

5′ATGTGAGACCCGTAATCGACCCTC

TCCGGTCCCTGGTCGATCCAATGAA

AATGGCTCCTAAGAAGAAGAGG

(TJ 5′ homologous sequence)

(FlpO 5′ sequence)

ATG: start codons in tj

Amplification of FlpO-lateSV40pA-kanR cassette to add homologous recombination arms to it

recTJantisens

5′ATTCATTAATTTAGATTTATTTAT

TACTAAATTGTTTTATGCACACTTA

TAACTCAGAAGAACTCGTCAAG

(TJ 3′ homologous sequence)

(KanR 3′ sequence)

Amplification of FlpO-lateSV40pA-kanR sequence to add homologous recombination arms to it