Fig. 6

The therapeutic effect on abscopal tumors with SMPAI strategy. a Schematic illustration of the animal experimental design for distal tumors. b Distal tumor growth curves with the mean tumor volumes of 4T1 tumor-bearing BALB/c mice model (n = 5 biologically independent samples). c Distal tumor growth curve of individual mouse in different groups of 4T1 tumor-bearing BALB/c mice model. d Representative flow cytometry plots showing different groups of T cells in distal tumors (gated on CD3⁺ T cells). e Representative flow cytometry plots showing percentages (gated on CD4⁺ cells) of Tregs in distal tumors on day 8 after different treatments. f Proportions of the intratumor infiltration of CD8⁺ killer T cells in distal tumors. Data represent mean ± SEM (n = 3 biologically independent samples). g The frequency of Tregs in distal tumors upon various treatments. Data represent mean ± SEM (n = 3 biologically independent samples). h CD8⁺ CTL/Treg ratios in the distal tumors after various treatments. Data represent mean ± SEM (n = 3 biologically independent samples). The comparison of two groups was followed by unpaired Student’s t-test (two-tailed). *P < 0.05, **P < 0.01, and ***P < 0.001