Fig. 2

RPM-1 inhibits UNC-51 to regulate axon termination. a Schematic of C. elegans mechanosensory neurons. Blue boxes highlight locations of ALM and PLM axons imaged in panels below by confocal microscopy. b Representative images of ALM axons for indicated genotypes visualized using transgenic GFP. Note failed termination (magenta arrow) in rpm-1 mutants compared with premature termination (orange arrow) in both unc-51 single mutants and rpm-1; unc-51 double mutants. c Representative images of PLM axons for indicated genotypes. rpm-1 mutants display severe failed termination defects (hook, magenta arrow), while rpm-1; unc-51 double mutants display less severe failed termination defects (overextension). d Representative images of premature termination defects observed in PLM neurons of unc-51 single mutants and rpm-1; unc-51 double mutants. e Quantitation of failed termination and premature termination defects in ALM neurons for indicated genotypes. Failed termination defects are suppressed in rpm-1; unc-51 double mutants, but not rpm-1; unc-14 double mutants. f Quantitation of failed termination and premature termination defects in PLM neurons for indicated genotypes. Severe hook defects are suppressed in rpm-1; unc-51 double mutants, but not rpm-1; unc-14 double mutants. Means are shown from 5 to 8 counts (25–35 animals/count) for each genotype, and error bars represent SEM. Significance determined using Student’s t test with Bonferroni correction. ***p < 0.001; ns, not significant. Scale bars 20 μm. Source data are provided as a Source Data file