Fig. 6

Differential expression of satellite prophage genes. The data were generated from pneumococcal reference strain Sp6A-10, which contains two full-length prophages (Spn_6A-10_FP1 and Spn_6A-10_FP2) and one satellite prophage (SpnSP33). Genes belonging to SpnSP33 are shown in green, while those belonging to Spn_6A-10_FP1 and Spn_6A-10_FP2 are shown in blue and magenta, respectively. Differential gene expression and statistical significance were computed using the DESeq2 method70. The dashed line above the x axis marks the significance threshold of p = 0.001. A higher log2 ratio denotes increased expression levels in planktonic growth as compared with growth in a biofilm. A full list of the genes depicted here, their expression levels and sequences may be found in Supplementary Data 5. The annotated genes and relative expression levels of all three prophages are found in Supplementary Fig. 6