Fig. 7
AZD7648 in combination with olaparib affects genome stability and induces apoptosis in ATM KO cells. a Frequency of micronuclei formation in FaDu WT and ATM KO cells following 72 h treatment. Data are shown as mean number of micronuclei per cell (mean ± SEM; n = 2; unpaired t-test, P ≤ 0.05 is significant). b Chromosomal aberrations in FaDu WT and ATM KO cells treated with olaparib (1 μM), AZD7648 (1 μM), or the combination for 48 h were detected by metaphase spread analysis. Chromatid breaks, chromosome breaks and chromosome fusions were quantified separately. Data are shown as mean aberrations per metaphase spread (mean ± SD; n = 3; paired t-test, P ≤ 0.05 is significant, 50 metaphase spreads/sample). c Caspase 3/7 activity of FaDu ATM KO and WT cells following 72 h treatment. Graph represents mean fluorescence levels normalized for total cell confluency and relative to the DMSO vehicle-treated control (mean ± SD; n = 3; two-way ANOVA, P ≤ 0.05 is significant)
