Fig. 2 | Nature Communications

Fig. 2

From: Chloroplastic metabolic engineering coupled with isoprenoid pool enhancement for committed taxanes biosynthesis in Nicotiana benthamiana

Fig. 2

Expression of Taxus-originated proteins and committed taxadiene production in N. benthamiana. a Accumulation of TS, T5aH and CPR in plants by immunoblot analysis after agroinfiltration. Leaves infiltrated with A. tumefaciens cultures harboring the empty vector were set as controls. b GC–MS analysis of hexane extracts from infiltrated N. benthamiana leaves at 5 dpi (days post-infiltration); upper panel is control and bottom panel is TS-expression leaves. Accumulation of taxadiene (the peak at the retention time 18.8 min) was detected both in TS-expression leaves and TS, T5αH and CPR co-expressing leaves. No taxadiene-5α-ol was detected in the TS, T5αH, and CPR co-expression leaves. c Accumulation of taxadiene in agro-infiltrated leaves at 2, 4, and 5 dpi. d Mass spectra profile of the compound at 18.8 min matched exactly with the taxadiene mass spectra. Data in c represent the mean of n = 3 biologically independent samples (closed circles) and error bars show standard deviation. The source data of a, c are provided as a Source Data file

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