Fig. 4
From: Identification and characterization of Cardiac Glycosides as senolytic compounds
Senolytic activity of Digoxin on xenografts. a Diagram showing the experimental plan to test the effect of administrating Gemcitabine, Digoxin or the combination of both (Gem+Dig) in the growth of A549 cells expressing luciferase (A549-Luc) as subcutaneous tumors in nude mice. b Tumor volume (mm3) progression with time after intraperitoneal injection of Gemcitabine (n = 12), Digoxin (n = 8) or the combination of both (Gem+Dig) (n = 12), or vehicle (n = 4) as negative control as indicated. Statistical analysis was performed with Anova with Tuckey test: ***p < 0.001; **p < 0.01. c Tumor growth determined by measuring normalized total flux of luminescence as detected by IVIS with the same groups of animals. Statistical analysis was performed with Anova with Tuckey test: ***p < 0.001. d Representative images of SABG staining (upper panels) and immunohistochemical analysis of p21 (middle panels) and Ki67 (bottom panels) in tumors obtained after injection of A549 cells in nude mice and treated with Gemcitabine, Gemcitabine plus Digoxin (Gem+Dig), or control group (scale bar = 50 μm). e Quantifications of stainings shown in (d). n = 3 biologically independent samples. Statistical significance was assessed by the two-tailed Student's t-test: ***p < 0.001; **p < 0.01; *p < 0.05. All data correspond to the average ± s.d. Source data for these experiments are provided as a Source Data file
