Fig. 5
Theabrownin directly reduced BSH activity to elevate ileal conjugated BAs. a Spearman correlation between Pu-erh tea constituents and OTUs of BSH-producing microbes in mice fed normal diet and HFD supplied with 450 mg/Kg/day Pu-erh tea for 26 weeks. n = 8 individuals/group. The color of each spot in the heatmap corresponds to the R value of the spearman correlation analysis between microbial abundance and tea components concentration, and the spot with asterisk in magenta color refers to the significant positive correlation with R > 0.3 and P < 0.05 while the spot with asterisk in green color refers to the significant negative correlation with R < −0.3 and P < 0.05. b Pu-erh tea and theabrownin reduced serum and hepatic TC and TG of mice. n = 8 individuals/group. c Ileal BAs changes in Pu-erh tea and theabrownin intervention in vivo. n = 8 individuals/group. d BSH-producing microbe changes induced by Pu-erh tea and theabrownin in vivo. n = 8 individuals/group. e Ileal BSH activity changes induced by Pu-erh tea and theabrownin in vivo. n = 8 individuals/group. f Theabrownin and Pu-erh tea reduced BSH activity in cultured ileal microbes. n = 8 individuals/group. Data were expressed as mean ± SEM. Differences between data were assessed using the Mann–Whitney U test, *p < 0.05, #p < 0.005
