Fig. 6
Conjugated BAs inhibited the FXR-FGF15 to induce hepatic BA synthesis. a Gene expression of FXR and FGF15 in ileum of mice fed HFD and 450 mg/Kg/day Pu-erh tea for 26 weeks. n = 8 individuals/group. b FXR and FGF15 protein expression level in ileum of mice fed HFD and 450 mg/Kg/day Pu-erh tea for 26 weeks. n = 3 individuals/group. c Gene expression of FXR and FGF15 in ileum of mice fed HFD and 225 mg/Kg/day theabrownin for 8 weeks. n = 8 individuals/group. d Serum FGF15 levels of mice fed HFD and 450 mg/Kg/day Pu-erh tea for 26 weeks. n = 8 individuals/group. e Serum FGF19 levels of human subjects that received standard diet and 50 mg/Kg/day Pu-erh tea for 4 weeks. n = 13 individuals/group. f Hepatic mRNA expression levels of BA synthetic enzymes of mice fed HFD and 450 mg/Kg/day Pu-erh tea for 26 weeks. n = 8 individuals/group. g Hepatic mRNA expression levels of BA synthetic enzymes of mice fed HFD and 225 mg/Kg/day theabrownin for 8 weeks. n = 8 individuals/group. h Hepatic protein expression levels of BA synthetic enzymes of mice fed HFD and 450 mg/Kg/day Pu-erh tea for 26 weeks. n = 3 individuals/group. i IHC staining of hepatic BA synthetic proteins of mice fed HFD and 450 mg/Kg/day Pu-erh tea for 26 weeks (scale bar, 50 μm). The mRNA expression and protein expression were normalized to GAPDH and β-Actin, respectively. Data were expressed as mean ± SEM. Differences between data in mice and human were assessed by Mann–Whitney U test and Wilcoxon rank-sum test, respectively, *p < 0.05, #p < 0.005
