Table 1 Complete list of antibodies used for this study

From: The transcription factor ERG regulates a low shear stress-induced anti-thrombotic pathway in the microvasculature

Antibody (host)

Company

Catalogue number

Application and dilution

CD31 (rabbit)

Abcam

ab28364

IF (mouse tissue, 1/200)

CD41 [MWReg30] (rat)

Abcam

ab33661

IF (mouse tissue, 1/200)

Isolectin B4 (IB4)

Vector

FL-1201

IF (mouse tissue,1/200)

Wheat Germ Agglutinin (WGA)

Thermo Fisher Scientific

W11261

IF (mouse tissue, 1/400)

ERG (mouse)

Santa Cruz

sc-376293

PLA (HUVEC, 1/200)/WB (HUVEC, 1/2000)

ERG (rabbit)

Abcam

ab133264

IF (HUVEC, 1/200)/WB (HUVEC, 1/1000)

ERG (rabbit)

Abcam

ab92513

IF (mouse tissue, 1/200)/WB (mouse tissue, 1/1000)

Fibrinogen (rabbit)

Abcam

ab34269

IF (mouse tissue, 1/200)

GAPDH (mouse)

Millipore

MAB374

WB (HUVEC, mouse tissue, 1/10000)

H3K27Ac (rabbit)

Active Motif

39133

IF (HUVEC, 1/200)

KLF2 (rabbit)

Abcam

ab203591

PLA (HUVEC, 1/100)/WB (HUVEC, 1/500)

Actin, α-smooth muscle-Cy3 (mouse)

Sigma

C6198

IF (mouse tissue, 1/400)

TM (mouse)

Dako

Clone 1009

IF (HUVEC, 1/100)

TM (mouse)

Santa Cruz

sc-13164

WB (HUVEC, 1/1000)

TM (goat)

R&D

AF3894

IF (mouse tissue, 1/200)/WB (mouse tissue, 1/1000)

  1. List of antibodies used for immunofluorescence (IF), western blot (WB) and proximity ligation assay (PLA). Dilution of the antibodies used for each specific application is specified in the right column (in brackets)