Fig. 7 | Nature Communications

Fig. 7

From: An anionic, endosome-escaping polymer to potentiate intracellular delivery of cationic peptides, biomacromolecules, and nanoparticles

Fig. 7The alternative text for this image may have been generated using AI.

PPAA enhances endosomal escape and gene editing efficiency. Comparison of a Galectin-8 recruitment and b gene editing efficiency with various delivery systems with and without PPAA pretreatment (50 ng/mL); *p < 0.05. **p < 0.01; one-way ANOVA followed by a Tukey’s post hoc test. Data are presented as means ± SEM. Successful CRISPR/Cas9-mediated gene editing leads to removal of a termination cassette upstream of a tdTomato transgene in Ai9 fibroblasts, leading to activation of tdTomato expression. c Representative microscopy images of galectin-8-YFP recruitment to disrupted endosomes and tdTomato expression following CRISPR/Cas9-mediated gene editing in engineered Ai9 fibroblasts. All Gal8 micrographs shown are 458.6 × 458.6 µm; all Ai9 micrographs shown are 1404 × 1404 µm. 50 ng/mL = 2.27 nM PPAA

Back to article page