Fig. 7
From: Split intein-mediated selection of cells containing two plasmids using a single antibiotic
SiMPl plasmids increase production of the non-proteinogenic aromatic amino acid L-PAPA. a Schematic showing the de novo L-PAPA biosynthesis pathway in E. coli. Broken arrows indicate incomplete presentation of the metabolic pathway. Plasmid-borne overexpressed enzymes are colored. AroF, DAHP synthase; AroB, dehydroquinate synthase; AroL, shikimate kinase; GlpX, fructose-1,6-bisphosphate phosphatase; PabAB, 4-amino-4-deoxychorismate synthase; PapB, 4-amino-4-deoxychorismate mutase; PapC, 4-amino-4-deoxyprephenate dehydrogenase; PheA, bifunctional chorismate mutase/ prephenate dehydratase; TktA, transketolase A; TyrA, bifunctional chorismate mutase/ prephenate dehydrogenase. b Schematic showing the plasmids used in (c) and (d). c, d L-PAPA production in E. coli FUS4.7 R cells cultivated in flasks (c) or in a bioreactor (d). Optical density (OD600) is shown in white. L-PAPA concentration in violet and green. Circles, production from cells co-transformed with pC53BC and pJNT-aroFBL; Squares, production from cells co-transformed with pC53BC_SiMPlk_C and pJNT- SiMPLk_N; Vertical dotted line, time at which production is initiated by addition of IPTG (0.5 mM final concentration). Values represent mean (± standard deviation) of three independent experiments. Source data are provided as a Source Data file
