Table 2 Basic sample information showing nuclei numbers and correlation with bulk RNA-seq of the same sample.

Donor	Region	Number of nuclei	Number of nuclei after QC	Correlation with bulk
12–887	Macular	1621	1428	0.68
12–887	Peripheral	415	388	0.66
13–0025	Macular	1613	1458	0.69
13–0025	Peripheral	424	391	0.67
13–0347	Macular	1805	1669	0.69
13–0347	Peripheral	664	539	0.71

Calculation of correlation was performed between the single-nuclei RNA-seq and bulk RNA-seq of the same samples. For single-nuclei RNA-seq (3′-end seq), the gene expression data from each nucleus were gene-filtered, normalized, and log-transformed (method) before averaging. For bulk RNA-seq (paired-end seq), FPKM was used

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